FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001211477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13486510
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG1282270.95077970505342TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT989240.733503330364935TruSeq Adapter, Index 7 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAACTCGTATG295950.21944150117413622TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)