FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4821839
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT720461.4941602156355698TruSeq Adapter, Index 7 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG382810.7939087140819093TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAACTCGTATG158190.3280698505279832TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)