FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001211499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16538282
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2689311.6261120713747654TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG2002451.2107968651157357TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAACTCGTATG302580.18295733498799935TruSeq Adapter, Index 19 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC289050.17477631594382054TruSeq Adapter, Index 19 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)