FastQCFastQC Report
Fri 10 May 2024
EGAF00001211505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449205
Total Bases516.4 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG1849125.361003477612957TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT433231.2560285631036716TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTATG425481.233559617361102TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCCATGATCTCGTATG95480.2768174115484583TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTATG63700.18468023790989518TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTATG60230.17461994865483496TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTATG51120.148208065336795TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTTTGATCTCGTATG36030.10445885356190775TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)