FastQCFastQC Report
Thu 5 Jan 2023
EGAF00001211506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17038115
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT15903699.334183975163919TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG1006470.5907167547583756TruSeq Adapter, Index 19 (97% over 37bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT536440.31484703560223654TruSeq Adapter, Index 12 (97% over 35bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT295480.17342294027244212TruSeq Adapter, Index 12 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT249360.14635421817495656TruSeq Adapter, Index 12 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAACTCGTATG187940.11030562946663994TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)