FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001211509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15652030
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2349961.5013771376620157TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG1079890.6899360658010495TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAACTCGTATG177480.11339104256764139TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)