FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616096
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG2714337.506244303248587TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAACTCGTATG649151.795168048635877TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT636821.7610705025530293TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGCATCTCGTATG315450.8723496278859852TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCACCTCGTATG178690.49415170393706365TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATTCGCATCTCGTATG133590.3694315637637939TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGCAACTCGTATG60910.16844132456660443TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTCGCATCTCGTATG58670.1622467987575551TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAGCTCGTATG49270.13625191366600886TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAAGCGCATCTCGTATG40020.11067184057060432TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATATCGTATG39460.10912320911834199TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCACCTCGTAT36840.10187782625240037TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)