FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470304
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG3260609.395718645974531TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT922612.6585855302590207TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTATG710182.0464489566331943TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCCATGATCTCGTATG217350.6263140059199425TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTATG106030.305535192305919TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTATG100680.2901186754820327TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTTTGATCTCGTATG92770.2673252833181185TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTATG87840.2531190351047055TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTCTGATCTCGTATG71590.2062931662471069TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT60920.17554658035722517TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCCATTATCTCGTATG58950.16986984425571938TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATTATCTCGTATG56230.1620319142069398TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCCATGAACTCGTATG53300.15358884985292354TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTTTCATCTCGTATG39540.11393814490027387TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATCATCTCGTATG36120.1040831005007054TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)