FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186197
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG905402.162822246540237TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT305000.7285849184832915TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAACTCGTATG194500.46462218572131225TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCCATCTCGTATG175760.4198560172872896TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCCAACTCGTATG44770.10694671082130153TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)