FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4045101
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG722281.7855672824980142TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT404010.9987636897076241TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAACTCGTATG292420.7228991315668014TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGCATCTCGTATG189520.46851734975220644TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCACCTCGTATG86240.2131961599969939TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGCAACTCGTATG64100.1584632868252239TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)