FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4697147
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG648631.3809020667226297TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT385790.8213283510181819TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAACTCGTATG141520.3012892719772236TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCCATCTCGTATG119820.2550910158868777TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)