FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794340
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG689401.437945577493461TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTATG595511.2421104886178287TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT249050.5194667044890433TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)