FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5500769
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG708101.2872745610659164TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTATG308810.5613942341516249TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT208070.3782562038144121TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGCGCATCTCGTATG179060.32551812301152805TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTATG110830.20148092021315564TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCCCGCATCTCGTATG99020.1800111947984TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGCGCAACTCGTATG90400.16434065855155888TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)