FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001211539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896539
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT58979820.36216325759812TruSeq Adapter, Index 13 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2224267.679026590009663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT122050.4213649462341091TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAACAATCTCGTAT100610.34734557345853107TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCCACAATCTCGTAT99450.34334079396134487TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT88860.3067799190689302TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT76970.2657309292227724TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAACAATCTCGTAT69230.23900938326740984TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAC59200.20438185020122293TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCAACAATCTCGTAT48560.167648355502895TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCCACAATCTCGTAT43610.1505589947174887TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCGCGTAT42390.14634707145320675TruSeq Adapter, Index 13 (97% over 40bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT35690.1232160174608386TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAACCTCGTAT34420.11883147439064345TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCAACAATCTCGTAT34200.1180719472446254TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACACTCTCGTAT34000.11738146802097263TruSeq Adapter, Index 13 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTAT33770.11658741691377192TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC30510.1053326055682316TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGGCTGAACTCCAGTCACAGTCAACAATCTCGTAT30310.10464212634457883TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACAGTCAACAATCTCGTAT30270.10450403049984827TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)