FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001217074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001217074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39563285
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5693601.4391120454229216Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTATGCCG1020000.2578147896465119Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)