FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001221529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001221529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8048765
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296280.36810616287094977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276100.3430339934138964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161930.20118614470667237No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA93210.11580658647631034No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88790.11031506075776844Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG80510.10002776823525099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)