FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001227608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001227608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660164
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA129740.3544649911861873No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA105790.28903076474168915No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT89230.24378689042348925No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC80050.2187060470514436No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG79010.21586464431648417No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA75490.20624758890585232No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA75160.2053459899611056No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA73610.20111120703881027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71820.19622071579306283No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG70520.19266896237436357No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG68350.18674026628315016No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG66840.18261476808143023No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT65310.17843462751942263No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA65290.17837998515913495No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA62870.17176825956432554No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC61410.16777936726332482No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT60170.16439154092548858No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT58010.15849016601441904No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG54540.14900971650450634No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT49700.13578626531488752No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC49330.13477538164956543No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC49010.13390110388496254No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA47830.13067720462798935No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC47290.12920186090022195No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA46440.1268795605879955No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA46000.12567742866166653No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA43630.1192023089675763No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT43360.11846463710369262No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT42020.114803598964418No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT41970.11466699306369879No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA40350.11024096188039662No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG40200.109831144178239No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC39970.10920275703493068No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG39920.10906615113421146No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT39450.10778205566745096No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT38080.10403905398774481No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC36860.10070587001019626No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA36760.10043265820875785No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)