FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001270747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001270747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4802
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTGACTGCCTCTTCCAGGGCGGGCGGTGTGGTGCACGCAT80.16659725114535612No Hit
GTACTGATGTGGTTCAGAGAGATAAAGCACTTTAGTACATAGTCACTCTT80.16659725114535612No Hit
CTCCTAGACAGTCACTTCGGTCCCTGGATAATGCATGTAAACTAATGGTG70.1457725947521866No Hit
GGATAATGCATGTAAACTAATGGTGGGGTATTCCAGTGAGAATCCTAATC70.1457725947521866No Hit
GAGAAATACTTTCCCTCTTGTCTTTTTTTCTCCCCCATCTCTCTTTTTTT70.1457725947521866No Hit
CTTTAGTACATAGTCACTCTTTTTAGTACAAACAAATCATTTGGAATACC60.12494793835901709No Hit
CATTTAGGCCTCATTTAGACAATGAGGAGGCTGAGCCTGTCCCTCCCTAA60.12494793835901709No Hit
ATAATAGATCCACGTTACTGGAATCTGTAAACCTTGAGGGATAGCTTTCT60.12494793835901709No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA60.12494793835901709No Hit
CTACTCACTGAGCTAGGATTAGGGAGGGACAGGCTCAGCCTCCTCATTGT50.10412328196584757No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT50.10412328196584757No Hit
AACTAATGGTGGGGTATTCCAGTGAGAATCCTAATCCAGAGCCATCTAAC50.10412328196584757No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC50.10412328196584757No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA50.10412328196584757No Hit
ATCCTAACCAGTATAATAAGCAAATACACTATAATAGATCCACGTTACTG50.10412328196584757No Hit
GCCTCAGTGTCTGGCTGCTGCCGCAGGAGCCCCTCCGCTGGCCCAGGCGG50.10412328196584757No Hit
GTCCCTGGATAATGCATGTAAACTAATGGTGGGGTATTCCAGTGAGAATC50.10412328196584757No Hit
GTACAGAGTGGGATGGTCAGGACCTGGGCCATTGCAACCAAAATGGGGAC50.10412328196584757No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC50.10412328196584757No Hit
GGGGATTTTTGTAGAGGCCAAACTGAGACCTTCAGCTGATGAACTCTTAA50.10412328196584757No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG50.10412328196584757No Hit
GGTCAGGACCTGGGCCATTGCAACCAAAATGGGGACTTCCTGGGTAGGGA50.10412328196584757No Hit
GCCAGACACTGAGGCTGTGCTGAACGGGAAGGGCCTCGGCACTGGTACCC50.10412328196584757No Hit
TCTGTATTCTTTAGATCCTAACCTCTGTCTACCAACCTTTTTGCTCAGTA50.10412328196584757No Hit
GTGTCTGGCTGCTGCCGCAGGAGCCCCTCCGCTGGCCCAGGCGGCGGGAA50.10412328196584757No Hit
GTACATGGGGGATTTTTGTAGAGGCCAAACTGAGACCTTCAGCTGATGAA50.10412328196584757No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)