FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001270994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001270994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTATTCATTCTGCTTTATTCTTCTAGGTAGTTTTTTTACTGAATC21.1904761904761905No Hit
GATGTCACTTATTTATTTCAATAAATATATCGCCTAAAAACAACGCGGGG21.1904761904761905No Hit
GGGACTTAGCTCTCAGGAGCAGCAGCAGCTGTCTAGCCAAGGCTGACTAA10.5952380952380952No Hit
CATCAAGTGGGCATAAGCCAATTATGCTGCTGGAAATCCTACAATGCACA10.5952380952380952No Hit
CAAAAAAAATCACACTTTAGTTTTTAAGTTTTAACAGATGTATTTCAAAT10.5952380952380952No Hit
ATCTAATACAATTCTTTTGTTATTTTGCATTCAGAATAGGTTGCAGCTCT10.5952380952380952No Hit
TACCTGTACTTTATTCCTTTTTATTGTTGGATAATATTCCATTGTATGGC10.5952380952380952No Hit
TTCTGGAAGGGAGGGAGACAAGCACAAGCTGAAGAAATCCGAACTCAAGG10.5952380952380952No Hit
CCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCT10.5952380952380952No Hit
GTACATGGGAGTTGGGCATGTTGACAGACATGTTTCGCCTCCCCCCTCCC10.5952380952380952No Hit
GTACATGGGTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGA10.5952380952380952No Hit
GTCTTCGGCAGGTTACAGGAGTTACTAGAGTCACTATCCGGAAATCTAAG10.5952380952380952No Hit
ATGGTAAATATCTATGTTCAAAGTCCATTGATGCTTTGAGATTCTGAGCT10.5952380952380952No Hit
CCCTCTGACAGTCCCTGGTGTGTGATGTTTCCCTCCTTGTGTCCATGCTG10.5952380952380952No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGCAA10.5952380952380952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5952380952380952No Hit
GACCCTGGGAGTCGGCCATCATGCTGGCCCCGGAGGCCGTTAGCTTAGTC10.5952380952380952No Hit
TCTTTACATCATCTTCCCTTTGTATGTGTCTATGTCCAAATTTTTCTATT10.5952380952380952No Hit
GAGTACATGGGGCGGTGGTTCAAGATGGCGGCGCCCAGTGGCACAGTGAG10.5952380952380952No Hit
CCTTTGTATATGCAATTCCCTCTGTCTGGGACACTCTTCCTGTGACTAAC10.5952380952380952No Hit
ACACTGAACTGGGGAACATGGCTTGGGGCCTCCAGGACAGTTCAGGTCCC10.5952380952380952No Hit
GTGCAGCTTAATTTCAATTCAGTCTTTACTTTGCCACTAGAGTTGAAAGA10.5952380952380952No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT10.5952380952380952No Hit
GCTCTCTTCTCAGTGTTTCAGCCCCGCTGCCCTGCTCACCTCTTGACCCT10.5952380952380952No Hit
CACAGCACACATAAGTAAGTTTTTAGCAATTCAAGATGTGGGAAGGCCAC10.5952380952380952No Hit
GTATCAACGCAGAGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT10.5952380952380952No Hit
GCGCTATGCCTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGG10.5952380952380952No Hit
GTGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCGCCTGCCTC10.5952380952380952No Hit
CACCATCTTCCTCTGTGTTTCCTTTTGCTTTCTTTAACAGCTCCTCAGTC10.5952380952380952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT10.5952380952380952No Hit
GTACATGGGATGCGGGACATGAAAAAACAGTGCTTCAAGCTCCTCAAGCC10.5952380952380952No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT10.5952380952380952No Hit
GAGTAGAGGAGCGTCTACTGGTCTTTCAACTCCTTCAGTCTTCTGATGGC10.5952380952380952No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT10.5952380952380952No Hit
AACCAGGGCTAGGCGTACACATCGGTCTTTGTTAAGACGGTCATCTTTGA10.5952380952380952No Hit
GCTCTGTTTGTGGCCATGATTACCTTGACATTGACATTCTGATCAAATCC10.5952380952380952No Hit
ACAATAAATAAATCTTTATTAGCTGTTCAATAAAGCCAGCAGTGGACGTC10.5952380952380952No Hit
GTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCT10.5952380952380952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCCAGCAA10.5952380952380952No Hit
ACGCAGAGTACATGGTGAAGCCCCGTCTCTACTGAAAATACAAAAATCAG10.5952380952380952No Hit
AGCCTTTCCTGTGCCCGCACTGTGGCCGGGCGTTTCGTCAGCGGGGCAAC10.5952380952380952No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC10.5952380952380952No Hit
CTTTCTGTATATAAGCTCTTTAACTGCTATTTTAGGGACCTTGCAGTTTG10.5952380952380952No Hit
CTGTCGCCCAGGCTGGAGTGCAGTGGCGCAATCTCAGCTCACTGCAACTT10.5952380952380952No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC10.5952380952380952No Hit
CTCGACGCTATCGGAGCCGTGGCAGACGTTGCGGCCGACATCCTAAAACT10.5952380952380952No Hit
GTAACAAATTATACTTAGAATTATTATGGCCAACTGGCTATGCCTTTCAG10.5952380952380952No Hit
GGTATCAACGCAGAGTACATTTTTTTCGCGGAGAGGTGCGCCTCGCCAGG10.5952380952380952No Hit
GTACATGGGGCTTCCTTTTCGATCCGCCATCTGCGGTGGGTGTCTGCACT10.5952380952380952No Hit
ATAACATATAGATCCAAAAAATTAATCCAAGAATCGGATCAGCACCTGAA10.5952380952380952No Hit
GTTTTATCAGAGACTAGGATTGCAACCCCTGCTTTTCCTTTTTTGCTTTC10.5952380952380952No Hit
ACTGAGGAGGCCGCGGCAGGGGAATCACTTCAACTCGGGAGGATGAGGTC10.5952380952380952No Hit
GTGCTCCCGTTTCTTTCTCCTTCTGAAAGCGGATCTGTAGCTGTTTGATC10.5952380952380952No Hit
ACCTTAAGCACACCTCGAGTCCCTCATAGATAAGACCGGAAATGCGCTTG10.5952380952380952No Hit
GTACTCAGGGACCCACCACTACACAGCTGTACAGCAAGGGAACCACAGTA10.5952380952380952No Hit
CAACTATACTAGGCTTTTTCTTGGTCTTTGGAAGCAATGACGACTTCAGC10.5952380952380952No Hit
TATCCATCCTTCTTAAGGATAATCCAGCTATGTTCAGCTTTACAGAAAGA10.5952380952380952No Hit
GTACATGGGGGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCT10.5952380952380952No Hit
CGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGC10.5952380952380952No Hit
GCACTGAGAACAACGTCAGCTCTCCTGGTTTTCCTGTCACTGGTAGACCC10.5952380952380952No Hit
CTACCGAGTCTGGTGGCGTGGGCTGCTAAATCTTTTCGCGGAAAGATTGT10.5952380952380952No Hit
CTACACAACTGGTCAGAAACTGTTCTAGGGATACAAAGCGGGTAAGATAA10.5952380952380952No Hit
GGTCTGCCCACCTTGATGTTCCTGCCCCCCAGCATCACCGAGTTCATCTG10.5952380952380952No Hit
GTGCTGATTGGTGCATATACAATCCTCCAGCCAGACATAAAAGTTCTCCA10.5952380952380952No Hit
GCCTGGACTGCAGTGCCTCCAAATCAACACGCAGCAAGAGGGGAGTGCAG10.5952380952380952No Hit
GGTCTTCCACTACTTTGTGAGCGTCCATGTCTCTGACATTGGAGTCACTT10.5952380952380952No Hit
CAAAAATGGACTGAAAAATAAGAAACAACAAACCTATGTAGGTTTTATAA10.5952380952380952No Hit
CTTTTGTTCTCTGCGTGAGGCTAAACTAGCTTCAGTTTCTTCACTAAGGA10.5952380952380952No Hit
GGCCATACATGTCTTACATAGGGACTCTGTTCAATTCATTAATACCTCCT10.5952380952380952No Hit
GTTGAAGGCCCGTGCCTCCTCAGCCAACTGGTCTATGATGGGCTGGGCCT10.5952380952380952No Hit
CTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCT10.5952380952380952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTT10.5952380952380952No Hit
GTGTATTCTAGATCATCTAAGATCCATGCTGATTTTTATTGCACAAGAAT10.5952380952380952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCTGAGAT10.5952380952380952No Hit
ATATTACTGATTACTGTTGTAAGCGCATTGTTGTGGTTAGAAGAGACGGA10.5952380952380952No Hit
CTGGGTGACAGACCGAGACTGTCACAAAACAACAAACCAAAAACCAAAAA10.5952380952380952No Hit
GTGTGACCACGACCGTGACTGTGCAGATGGCTCTGATGAGTCCCCCGAGT10.5952380952380952No Hit
CCCCTCTCGCTCCCTTTTAAAATCAGTGGCACCGAGGCGCCTGCAGCCGC10.5952380952380952No Hit
GTGCCGAGTAGAGGGAGGCTCGCCGAGCATGGGACGGGGAGGGCACTGCC10.5952380952380952No Hit
ACGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACCC10.5952380952380952No Hit
GCACAACAGAAAGCAGGAAGAACTATTACAGCCCGGATCACAGGAAGATG10.5952380952380952No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT10.5952380952380952No Hit
CTCCAAAAAAAAAAGAAAAAGTAACAAAAGGAAATTATTTGTTTTTGAAA10.5952380952380952No Hit
TTCTAAAATACTGTTTCTCATTTGCTGGGAAGCTCCAAAATACTAGTCCT10.5952380952380952No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5952380952380952No Hit
ATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAG10.5952380952380952No Hit
GCATTGCAGGTCGATAACCTCCATCTGGAGCTCCTCGTGCACACTGTCGA10.5952380952380952No Hit
CTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGAC10.5952380952380952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGTATGAAATTTTCTTTCTCCA10.5952380952380952No Hit
CAAGGGATGTGCTCCAAATCTCACAAAATCTGGTCTCCTCTTTCTCTAGG10.5952380952380952No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC10.5952380952380952No Hit
ATTCTAATACAGTAAGATATTGGACACAAAATGGAGGTAACTTTTTAAAA10.5952380952380952No Hit
GTCCCTTCAGAGACCCACAGGCCGGTGGCATCGGCGCGGAGTGCCCAGGT10.5952380952380952No Hit
ATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAAT10.5952380952380952No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC10.5952380952380952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTCGCTTTTC10.5952380952380952No Hit
TCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGG10.5952380952380952No Hit
GGCCTGCACCACGGGCTCGTCCGGGTCGGGTGCCAGCACCGCGACGGCTC10.5952380952380952No Hit
GTATTTTGTGTGCCTGAAATATTTCATAATTTTAAAAAGAAAAAAACCAC10.5952380952380952No Hit
GTGTGTGCCATCATCGAGCGTGTGCAGCGCTACCTGGAGGAGAAGGGCAC10.5952380952380952No Hit
GAGTAACTAAAAGTGCGGCGATTCTGCACATCGCCGACTGCTTTGGGGTA10.5952380952380952No Hit
GCCTCAGAGGCTGCTGTCAGCGCATTGATCCGCACCATAGCTGGCTCCCA10.5952380952380952No Hit
CCACTATCTCGCCAAGGCTTGTCACTACATGCTCAGGCGCTGGGATGCGT10.5952380952380952No Hit
GTTTAATGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACA10.5952380952380952No Hit
AGGCCATGCTTGCACTCAGAAGTTTTCTCATGAGGAGATTGCCATGGCGA10.5952380952380952No Hit
TGGCAAGACCCTGTCTCCACAAAAAATACAAAAATTAGCCAGGTGTGGTG10.5952380952380952No Hit
CTGCTGACATTGTATGTAGAGAATGCCTTAGGGTTTTCTTCCAGAGTGTG10.5952380952380952No Hit
GCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCG10.5952380952380952No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT10.5952380952380952No Hit
GTGCCGTCCCTGACCTCCAGGCGCAGCGGACAGCGTGAGCCGCAATTAAC10.5952380952380952No Hit
GTATATGTGTGTAAAAAAGGATGTTTGGGCCGGGCATGGTGGCTCACGCC10.5952380952380952No Hit
GTGCTGAGAGGTGTAAGCCACCACGCCTGGTCCCACTGATATTTCTGCGG10.5952380952380952No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTCCAGCTTACTTCCATTTC10.5952380952380952No Hit
GGTTTCTTATTGACTCATTTCTTTACTCTACAAATATGAATTGAGTGACT10.5952380952380952No Hit
CCTAGGGCAAGTCATTTAACTCCTCTCCCTCTTAGGTTTCTCAGCTATAA10.5952380952380952No Hit
GTACTCCTTCAGCCGCTGCACGTTGGCCTGCAGGGACTCCGTGGACTTGT10.5952380952380952No Hit
GGGGATGGTGACCGGGATAGGAGGACGGGCTGGGGTCACACCTGTGATGA10.5952380952380952No Hit
GTATCAACACAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5952380952380952No Hit
GGCTAGCACAATTGAAATGCAATCCAGGCACAGGTTTACCACTACTACAA10.5952380952380952No Hit
GACCCGACGGCTCCGGCGGCTTCAGGATCGCCAGGATCTCCTCGGCCGAG10.5952380952380952No Hit
CCCCTGCCGCTCTCACCTCCCGGCCGCCTCCTGCGGCAGATCGCGGACCT10.5952380952380952No Hit
GGCCTGACGGCAACTGTTTCTATCGGGCTTTCGGACTCTCCCACTTGGAG10.5952380952380952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTCAAAAATTTT10.5952380952380952No Hit
GAATTACCTAGTACAGTGATATGTTAAATATAATAGACTTTATGGTAAAT10.5952380952380952No Hit
CTCGTGGACAAACCAAGAGTGGCAGATTGGTGATAGCTTAGCTGCTGTCT10.5952380952380952No Hit
GTCTTATGCCCTTAATGATGACAGCACAGGATCCTTCCAGTGCCCCAGAG10.5952380952380952No Hit
CTATGTGCCTGCTGATGACTTGACTGACCCTGCCCCTGCTACTACGTTTG10.5952380952380952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGG10.5952380952380952No Hit
GTATTAATCAATGAGGTAATCTTTGGTGTGCCAGCCTAGGCTCCAAAAGT10.5952380952380952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCATA10.5952380952380952No Hit
GCCGAATGCAGTGGCTCATGCCTGTAATCCCAACACTTTGGGAGGCCGAG10.5952380952380952No Hit
GTGCCCATCTACGAGGGCTACGCCCTCCCCCACGCCATCCTGCGTCTGGA10.5952380952380952No Hit
TGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTC10.5952380952380952No Hit
GAAGATAGTAAGATGATGATGTTGCCAAAAAAGGAAAAGGGGAGTGAGTG10.5952380952380952No Hit
GTACATGGGTTACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGTGAG10.5952380952380952No Hit
ATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATT10.5952380952380952No Hit
GAGTACATGGGGTAATCCTGGGGGCTTTGAGGCATGGGCTGGGGAGGAAA10.5952380952380952No Hit
CCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGA10.5952380952380952No Hit
TTTGCACCCAATGCTAAAGTTTTCAGGAGAAAGCATACTTAAGCCAATAA10.5952380952380952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5952380952380952No Hit
GTACATGGGAGTCCCAGCTACTCCGGAGGCTGAGGCAGGCGAATTGCTTG10.5952380952380952No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG10.5952380952380952TruSeq Adapter, Index 4 (95% over 23bp)
CTTGAAACTGAGTCCTCAGATCCACTGGTTTTTAGAAAGAATACCTGTAA10.5952380952380952No Hit
CCTGAAGCAGCCGTTTGTGCAGGGCCTGGCTCTCTATACCCCTGTGGTCC10.5952380952380952No Hit
GATTTTATAATTATGTGGCAGCCCTGTTGCTCTCTGAAGCTCACAGAGGC10.5952380952380952No Hit
GGTAGTCCTGCGGCCATGAGAGTGCCGCACATTCCCGCCAAAACGTCACC10.5952380952380952No Hit
CGTGCAGAACGTGCAGGTTTGTTACATAGGTATGCGTGTGTGCCATGGTG10.5952380952380952No Hit
TCTGATATCACTGTGGCTCAGTTCATGTGGATCATCAGGAAAAGGATCCA10.5952380952380952No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAACTTTTCAGGCGAATTTC10.5952380952380952No Hit
ATGTTAGCCAGGATGGTCTCGATCTGCTGACCTTGTGATCCGCCCGCCTC10.5952380952380952No Hit
CACTACTACACAGGGGCCTAGCACCCTCCAGCTTCCAGCAGAGCGAAGGG10.5952380952380952No Hit
CTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTAT10.5952380952380952No Hit
GGACAGGCATGGGCAGGGGTGGGGCAGCTGGCCCGGGAGGCCGGCAGGTC10.5952380952380952No Hit
GGGAACTTGGGCTTGGGGTTGGAGGGTCTGATTTTAGGTCTGGCGTCGAT10.5952380952380952No Hit
GTAGGTATTGGTGCCGCCGAGCTGCACGCCGAGCCTGTCTCTTATACACA10.5952380952380952No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT10.5952380952380952No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5952380952380952No Hit
GCATTAGAGAGATATGCAACATTCTCAGGTAACATTGTTTCTTTGGTTAC10.5952380952380952No Hit
CTCGTAAAGGATTTCCGCGTCCGTGTCGCAGGACAGACGTATATACTCCC10.5952380952380952No Hit
GGCCTGGCGAGAGCCGGCATCGCTGACAACCTGCAGCACCACGGTGATGG10.5952380952380952No Hit
ATGCTAGATATTCTGTCAAAGGGAATGGCAAGCCCCAGGGTCATGGGCTG10.5952380952380952No Hit
TCTCCGAGCCCACGAGACCTCTCTACATATCGTATGCCGTCTTCTGCTTG10.5952380952380952TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGGCCTTTTGTTCATCCATGGGTTCACTG10.5952380952380952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTCCAGTTTTTTTTTTT10.5952380952380952No Hit
CTCCAGGACAGTTCAGGTCCCCAAGCTAACCCCCTACTTCCCAGACAGCT10.5952380952380952No Hit
GGCGGATCACCTGAGGTTGGGAGTTCAAGACCAGTCTGGCCAACGTGGTG10.5952380952380952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)