FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001272459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001272459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5307938
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCGAGCTCAACAATTTTAGTGAATTTATCATCGTCCACTTGAATCTG1509152.8431944758962895No Hit
AATTCATTTCAGCATTTATTGGTTGTATGAGAGTAGATAGAAAAAGACAA1322172.490929622764998No Hit
AATTCAAGGGGGTTTGCTCTATTTAATTAGGAATAAGGTCGATTACTGAT855871.6124340563133932No Hit
AATTCATTAGTAATAGTTACGCTGCGGCCTTTTACACATGACCTTCGTGA825001.5542758788817805No Hit
AATTCTGCCGTCACTGCATAAACCATCGGGAGAGCAGGCGGTACGCATAC635871.197960488611585No Hit
AATTCACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGAC619651.1674024828473881No Hit
AATTCTCTGACGAATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTT544831.0264437904135277No Hit
AATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAG539431.0162703482972106No Hit
AATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATT522600.9845631203680224No Hit
AATTCACAACCTGACCCAGCAAAAGGTACACAATACTTTTATATTGGACT522370.9841298070926978No Hit
AATTCGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAA471120.8875763055257992No Hit
AATTCGAATAAACCAATGGACAGAATCACCGATTCTCAACTTAGCGAGAT369630.696372112861906No Hit
GGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGG341580.6435267329799256No Hit
AATTCAGGACAGACAGTGGCTACGGCTCAGTTTGGGTTGTGCTGTTGCTG312920.5895321309329536No Hit
AATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAAC297880.5611972106682482No Hit
AATTCAGATCTCCCTCAAACTGGGGGATTGCTATCCCTCAAAACAGGGGG217370.4095187245970093No Hit
AATTCTCTGACGAATAATCCTTTCTTTTTTCTTTTGTAATAGTGTCTTTT211710.3988554500824991No Hit
AATTCAGATCTCCCTCAAATTGGGGGATTGCTATCCCTCAAAACAGGGGG165510.3118160008651194No Hit
AATTCACCGGAAGCAGAACCGGATCACCAAATGCGTACAGGCGTCATCGC147420.27773496977545703No Hit
AATTCTCTAGACCGGCGCGTGAGTTCACCATGATTAGTCAGCACCGCTGA138000.2599879651947706No Hit
AATTCAATCCAGTCTGTGTTTGTCAGGTCGAGTTTTGGTTTGCTGGCTGT133180.25090722612057637No Hit
AATTCGAGCTCGGTACCCCTCGGTACCACCATCGCAGGCAGAGAGGTCTG124910.23532678791651296No Hit
AATTCATTACGCCAGTTGTACGGACACGAACTCATCAGGGTCAGCCAGCA124500.23455435990397777No Hit
AATTCCGGCGAATCCTCTGACCAGCCAGAAAACGACCTTTCTGTGGTGAA101380.1909969558800423No Hit
GGGTCACGTTCGATAATCGACATACCTTCGTGGAACACCGCAGCGCGAAT97540.18376250815288347No Hit
GGGGTGCTCGCGATATGCTCTACCCGGAGCGTATTTATCAGGAATTTGAA87440.1647344034538459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)