FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001272471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001272471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5317126
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCGAGCTCAACAATTTTAGTGAATTTATCATCGTCCACTTGAATCTG1522352.8631068739014274No Hit
AATTCATTTCAGCATTTATTGGTTGTATGAGAGTAGATAGAAAAAGACAA1345912.531273473677321No Hit
AATTCAAGGGGGTTTGCTCTATTTAATTAGGAATAAGGTCGATTACTGAT861811.620819216998055No Hit
AATTCATTAGTAATAGTTACGCTGCGGCCTTTTACACATGACCTTCGTGA840711.5811361250419869No Hit
AATTCTGCCGTCACTGCATAAACCATCGGGAGAGCAGGCGGTACGCATAC644941.212948498869502No Hit
AATTCACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGAC623391.1724190850470724No Hit
AATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAG549881.034167706388752No Hit
AATTCTCTGACGAATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTT545901.026682459659598No Hit
AATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATT531040.9987350309170782No Hit
AATTCACAACCTGACCCAGCAAAAGGTACACAATACTTTTATATTGGACT529430.9957070793507621No Hit
AATTCGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAA473480.8904810606331316No Hit
AATTCGAATAAACCAATGGACAGAATCACCGATTCTCAACTTAGCGAGAT375320.7058700508507791No Hit
GGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGG341980.6431670041296746No Hit
AATTCAGGACAGACAGTGGCTACGGCTCAGTTTGGGTTGTGCTGTTGCTG314040.5906198198049096No Hit
AATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAAC292610.5503160918135097No Hit
AATTCAGATCTCCCTCAAACTGGGGGATTGCTATCCCTCAAAACAGGGGG218940.4117637987138164No Hit
AATTCTCTGACGAATAATCCTTTCTTTTTTCTTTTGTAATAGTGTCTTTT210460.395815333321046No Hit
AATTCAGATCTCCCTCAAATTGGGGGATTGCTATCCCTCAAAACAGGGGG169670.3191009579235098No Hit
AATTCACCGGAAGCAGAACCGGATCACCAAATGCGTACAGGCGTCATCGC147530.27746192209851717No Hit
AATTCTCTAGACCGGCGCGTGAGTTCACCATGATTAGTCAGCACCGCTGA138580.2606295205342134No Hit
AATTCAATCCAGTCTGTGTTTGTCAGGTCGAGTTTTGGTTTGCTGGCTGT136820.25731946167911013No Hit
AATTCATTACGCCAGTTGTACGGACACGAACTCATCAGGGTCAGCCAGCA125860.23670682244505775No Hit
AATTCGAGCTCGGTACCCCTCGGTACCACCATCGCAGGCAGAGAGGTCTG125400.23584169342611028No Hit
AATTCCGGCGAATCCTCTGACCAGCCAGAAAACGACCTTTCTGTGGTGAA103050.1938077073968155No Hit
GGGTCACGTTCGATAATCGACATACCTTCGTGGAACACCGCAGCGCGAAT100060.18818436877365705No Hit
GGGGTGCTCGCGATATGCTCTACCCGGAGCGTATTTATCAGGAATTTGAA89970.1692079518145705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)