FastQCFastQC Report
Fri 6 Jan 2023
EGAF00001272909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001272909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131767567
Sequences flagged as poor quality0
Sequence length101
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA1689365412.820798307674606TruSeq Adapter, Index 19 (97% over 40bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA6080230.46143600723841244TruSeq Adapter, Index 19 (97% over 40bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA6060920.4599705479877305TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT4188750.31788930275991206TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGAAACGATCTCGTA2713680.20594445672659345TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGAAACGATCTCGTA2342130.17774707792851635TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGAAACGATCTCGTA2300900.17461808337100132TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGAAACGATCTCGTA2289370.17374305772831034TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTA2254500.17109673126164648TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGAAACGATCTCGTA2089480.15857316391066095TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTA1930800.14653074682634157TruSeq Adapter, Index 19 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGAAACGATCTCGTA1846320.14011945746861973TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTA1634850.12407074344781671TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCATACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA1598130.1212840182440342TruSeq Adapter, Index 19 (97% over 40bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA1371080.10405291918306422TruSeq Adapter, Index 19 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)