FastQCFastQC Report
Sun 25 Dec 2022
EGAF00001272910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001272910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131767567
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC93548917.099539904231516Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGCGTAGATCTCGGTGGTCGCC2482580.1884059982681474Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGCCGCC2256890.17127811125176198Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGCGGTCGCC2060810.1563973629413678Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGCAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1663950.1262791776370888Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGAAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1388060.10534155191618587Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1322090.10033500884174328Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)