FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001273033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001273033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16308163
Sequences flagged as poor quality0
Sequence length53
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT9121225.593039510336021TruSeq Adapter, Index 1 (100% over 53bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGCCGT545960.3347771297110533TruSeq Adapter, Index 1 (98% over 53bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAAT362680.22239169426991867No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCA213240.13075660330351127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)