FastQCFastQC Report
Sat 13 May 2023
EGAF00001273194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001273194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19875148
Sequences flagged as poor quality0
Sequence length100
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC965170.48561650962297237No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC890330.44796144411100736No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA678700.34148173387186853No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG555160.27932370616812513No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA552450.2779601943089933No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC536120.26974390329068243No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG532990.2681690722504306No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC530070.2666999008007387No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA508160.2556760835189755No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC503510.253336478299432No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT490250.2466648298669273No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA485380.24421453364774945No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC476620.2398070192986739No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA472760.23786489539599906No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC463130.23301964845746054No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG462610.23275801518559763No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT430180.2164411555576844No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA429030.2158625435141414No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA409340.20595569904686997No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC404800.20367143932714366No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT401320.20192050896929173No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT398800.2006525938825713No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA385840.1941318877222952No Hit
CATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTA378470.19042373923454556No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA370620.18647408311123018No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC359150.18070305690302282No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA355880.1790577861357309No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG353480.177850247957902No Hit
GTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAA345190.17367920983531795No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG344440.17330185415474642No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG337090.16960376848514536No Hit
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA335410.16875849176066512No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG334880.1684918270797279No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA330330.16620253595092727No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT322960.16249438746317763No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATG321170.16159376523888022No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC306120.1540214945820781No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGC302760.1523309411331176No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT301950.15192339699810034No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC299060.15046931977563135No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA298170.1500215243680198No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA297550.14970957700541399No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC292520.14717877824104755No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT290130.14597627147229295No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA288250.14503036656632695No Hit
CTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCC277120.13943040826664535No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC275050.1383889065882679No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTC270220.13595873600538724No Hit
ATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG268880.13528452718943274No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT265620.13364428783121515No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC265280.13347321992268937No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG260250.13094242115832294No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC260200.13091726411295151No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG260100.13086695002220863No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC255380.1284921249391451No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC253400.12749590594243626No Hit
CTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCC244850.12319405118392075No Hit
ATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGG239060.12028086532990849No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA238660.12007960896693701No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC237630.11956137383228543No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA236770.11912867265189674No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA235590.11853496638113084No Hit
CACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTT234400.11793622870129067No Hit
CAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGC228550.1149928543928327No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA227010.11421801739539249No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG226790.11410732639575816No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTC222430.11191363203936897No Hit
AATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGA220730.11105829249674014No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT219680.11052999454394001No Hit
CGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCC217190.10927717368444251No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC216290.10882434686775666No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA215690.10852246232329944No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG215220.10828598609680794No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT215100.10822560918791649No Hit
CCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGT214510.10792875605253355No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG214090.1077174368714135No Hit
CACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTAC212420.10687719155600753No Hit
CATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGAT211800.10656524419340171No Hit
ATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCG211500.10641430192117313No Hit
GGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCC211150.10623820260357306No Hit
ATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC210150.10573506169614436No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA209220.10526714065223564No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA208320.10481431383554979No Hit
ATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCC208100.10470362283591549No Hit
GTTACTCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACAT207970.10463821451794976No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT203690.10248477143415485No Hit
CGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA203080.10217785548062334No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC202170.1017199972548632No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG201250.10125710762002879No Hit
CACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA199590.10042189371369711No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC199470.10036151680480568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)