FastQCFastQC Report
Wed 22 May 2024
EGAF00001273249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001273249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129020500
Total Bases12.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC29367652.276200293751768TruSeq Adapter, Index 3 (100% over 50bp)
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA4247610.32921977515201073No Hit
TTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTT4044480.3134757654791293No Hit
AGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAG3668880.28436411267976797No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG3635270.28175910029801465No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT3634820.28172422211974063No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG3188000.24709251630554832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)