FastQCFastQC Report
Fri 10 Mar 2023
EGAF00001273250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001273250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129020500
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7240340.5611774872985301Illumina Single End PCR Primer 1 (100% over 50bp)
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT4819920.3735778422808778No Hit
TAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTA4305230.33368573211233876No Hit
CCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC3886850.3012583271650629No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA3633350.281610286737379No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC3390190.26276366933936857No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC3029590.2348146224824737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)