FastQCFastQC Report
Fri 6 Jan 2023
EGAF00001274116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001274116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144228388
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1510477910.472819678189845Illumina Single End PCR Primer 1 (100% over 50bp)
AATAATACAACAACCACCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT20589201.4275414351854225Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCC2672600.18530332599987182Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCC2279420.15804239592555108Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCC2218960.1538504333834751Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGAGAAAGAGTGTAGATCTCGGTGGTCGCC2039300.14139380105946966Illumina Single End PCR Primer 1 (98% over 50bp)
AATAATACAACAACCACCAAAATCTACACTCTTTCCCTACACAACACTCT2034510.14106168890967566Illumina Single End PCR Primer 1 (95% over 24bp)
AGATCGGAAGAGCGTCATGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1845890.1279838196624648Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1828840.1268016668119455Illumina Single End PCR Primer 1 (98% over 50bp)
AATAATACAACAACCACCAAGATCAGAAGAGCGTCGTGTAGGGAAAGAGT1773000.12293002955839734Illumina Single End PCR Primer 1 (96% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGACGCC1662270.1152526228054355Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC1656100.1148248290759514Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCC1530350.10610601846288402Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCATCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1525420.10576419948616497Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1457960.10108689559783474Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)