FastQCFastQC Report
Tue 24 Jan 2023
EGAF00001274198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001274198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165120238
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC142511218.63075366933519Illumina Single End PCR Primer 1 (100% over 50bp)
AATAATACAACAACCACCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT28532781.728000174030757Illumina Single End PCR Primer 1 (100% over 31bp)
AATCAAAAAAACACACATCTAAACTCCAATCACCTTATAATCTCATATAC5208330.31542650756111434No Hit
AATAATACAACAACCACCAAAATCTACACTCTTTCCCTACACAACACTCT3550040.21499726762748492Illumina Single End PCR Primer 1 (95% over 24bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCC2349420.14228540537835221Illumina Single End PCR Primer 1 (98% over 50bp)
AATAATACAACAACCACCAAGATCAGAAGAGCGTCGTGTAGGGAAAGAGT2331610.14120679743690778Illumina Single End PCR Primer 1 (96% over 31bp)
AGATCGGAAGAGCGTCGTGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCC2014480.12200079314323663Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCC1973950.11954621819282989Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1813630.10983692986198336Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGAGAAAGAGTGTAGATCTCGGTGGTCGCC1805370.10933668833495747Illumina Single End PCR Primer 1 (98% over 50bp)
AATAATACAACAACCACCAAGATCGAAAGAGCGTCGTGTAGGGAAAGAGT1681790.1018524452465966Illumina Single End PCR Primer 1 (96% over 31bp)
AATAATACAACAACCACCAAGATCGGAAAAGCGTCGTGTAGGGAAAGAGT1672500.10128982493351299Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)