FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001274291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001274291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20632177
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCC16006377.757964658794853TruSeq Adapter, Index 20 (97% over 44bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAAT341040.16529520854730936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)