FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001275195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001275195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874428
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT373421.299110640447421TruSeq Adapter, Index 16 (97% over 40bp)
GGGTCACGTTCGATAATCGACATACCTTCGTGGAACACCGCAGCGCGAAT221010.7688834091513164No Hit
GGGGTGCTCGCGATATGCTCTACCCGGAGCGTATTTATCAGGAATTTGAA209550.7290146074279822No Hit
AATTCGAGCTCAACAATTTTAGTGAATTTATCATCGTCCACTTGAATCTG80310.27939471783603553No Hit
AATTCATTTCAGCATTTATTGGTTGTATGAGAGTAGATAGAAAAAGACAA74760.26008652851976116No Hit
AATTCATTAGTAATAGTTACGCTGCGGCCTTTTACACATGACCTTCGTGA53730.18692414629971596No Hit
AATTCAAGGGGGTTTGCTCTATTTAATTAGGAATAAGGTCGATTACTGAT53450.18595003945132738No Hit
AATTCACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGAC43540.15147361492443018No Hit
AATTCTGCCGTCACTGCATAAACCATCGGGAGAGCAGGCGGTACGCATAC42330.14726408175817937No Hit
AATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAG41230.14343723342522408No Hit
AATTCGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAA39260.13658369595620418No Hit
AATTCTCTGACGAATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTT39230.13647932736530538No Hit
AATTCGAATAAACCAATGGACAGAATCACCGATTCTCAACTTAGCGAGAT29430.10238558767170372No Hit
AATTCACAACCTGACCCAGCAAAAGGTACACAATACTTTTATATTGGACT29420.10235079814140413No Hit
AATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATT28930.10064611115672405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)