FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001275207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001275207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857011
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT361271.2645033568299178TruSeq Adapter, Index 16 (97% over 40bp)
GGGTCACGTTCGATAATCGACATACCTTCGTGGAACACCGCAGCGCGAAT226070.7912815176420392No Hit
GGGGTGCTCGCGATATGCTCTACCCGGAGCGTATTTATCAGGAATTTGAA210080.7353139347380881No Hit
AATTCGAGCTCAACAATTTTAGTGAATTTATCATCGTCCACTTGAATCTG77360.2707724961506974No Hit
AATTCATTTCAGCATTTATTGGTTGTATGAGAGTAGATAGAAAAAGACAA73340.25670184679022934No Hit
AATTCATTAGTAATAGTTACGCTGCGGCCTTTTACACATGACCTTCGTGA52610.18414349822244297No Hit
AATTCAAGGGGGTTTGCTCTATTTAATTAGGAATAAGGTCGATTACTGAT52580.18403849337646933No Hit
AATTCACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGAC43170.1511019733560704No Hit
AATTCTGCCGTCACTGCATAAACCATCGGGAGAGCAGGCGGTACGCATAC42370.1483018441301066No Hit
AATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAG41430.14501169228959915No Hit
AATTCGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAA39050.1366813078423569No Hit
AATTCTCTGACGAATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTT38810.13584126907456778No Hit
AATTCACAACCTGACCCAGCAAAAGGTACACAATACTTTTATATTGGACT30160.10556487181883445No Hit
AATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATT28770.10069964728872238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)