FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001275241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001275241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914041
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT429841.4750650385495605TruSeq Adapter, Index 16 (97% over 40bp)
GGGTCACGTTCGATAATCGACATACCTTCGTGGAACACCGCAGCGCGAAT228530.7842374215050509No Hit
GGGGTGCTCGCGATATGCTCTACCCGGAGCGTATTTATCAGGAATTTGAA209800.7199624164519305No Hit
AATTCGAGCTCAACAATTTTAGTGAATTTATCATCGTCCACTTGAATCTG83250.2856857539066884No Hit
AATTCATTTCAGCATTTATTGGTTGTATGAGAGTAGATAGAAAAAGACAA79490.27278270964615803No Hit
AATTCAAGGGGGTTTGCTCTATTTAATTAGGAATAAGGTCGATTACTGAT56860.19512422783344502No Hit
AATTCATTAGTAATAGTTACGCTGCGGCCTTTTACACATGACCTTCGTGA56180.1927906985522853No Hit
AATTCACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGAC43770.15020378917112012No Hit
AATTCTGCCGTCACTGCATAAACCATCGGGAGAGCAGGCGGTACGCATAC42610.14622306275031818No Hit
AATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAG41980.14406111650453785No Hit
AATTCTCTGACGAATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTT38810.1331827520614844No Hit
AATTCGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAA38700.1328052693836497No Hit
AATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATT30940.10617558229276802No Hit
AATTCACAACCTGACCCAGCAAAAGGTACACAATACTTTTATATTGGACT30490.10463133497435348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)