FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001275267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001275267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901054
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT406851.4024213268694756TruSeq Adapter, Index 16 (97% over 40bp)
GGGTCACGTTCGATAATCGACATACCTTCGTGGAACACCGCAGCGCGAAT225940.7788203873488739No Hit
GGGGTGCTCGCGATATGCTCTACCCGGAGCGTATTTATCAGGAATTTGAA207330.7146712884351688No Hit
AATTCGAGCTCAACAATTTTAGTGAATTTATCATCGTCCACTTGAATCTG84170.29013592990685455No Hit
AATTCATTTCAGCATTTATTGGTTGTATGAGAGTAGATAGAAAAAGACAA76340.2631457394450431No Hit
AATTCAAGGGGGTTTGCTCTATTTAATTAGGAATAAGGTCGATTACTGAT55690.1919647135144675No Hit
AATTCATTAGTAATAGTTACGCTGCGGCCTTTTACACATGACCTTCGTGA55380.190896136369747No Hit
AATTCACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGAC45260.15601226312919372No Hit
AATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAG43810.15101407971033975No Hit
AATTCTGCCGTCACTGCATAAACCATCGGGAGAGCAGGCGGTACGCATAC42590.14680871159240744No Hit
AATTCTCTGACGAATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTT39320.13553694622712986No Hit
AATTCGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAA38440.13250356594534263No Hit
AATTCACAACCTGACCCAGCAAAAGGTACACAATACTTTTATATTGGACT30250.10427244718643638No Hit
AATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATT29910.10310045935029132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)