FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001275307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001275307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968927
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT386441.3016150279208616TruSeq Adapter, Index 16 (97% over 40bp)
GGGTCACGTTCGATAATCGACATACCTTCGTGGAACACCGCAGCGCGAAT240800.8110674327795867No Hit
GGGGTGCTCGCGATATGCTCTACCCGGAGCGTATTTATCAGGAATTTGAA218870.7372023630085887No Hit
AATTCGAGCTCAACAATTTTAGTGAATTTATCATCGTCCACTTGAATCTG83870.2824926311761791No Hit
AATTCATTTCAGCATTTATTGGTTGTATGAGAGTAGATAGAAAAAGACAA77970.26262013178498494No Hit
AATTCAAGGGGGTTTGCTCTATTTAATTAGGAATAAGGTCGATTACTGAT55250.1860941680277083No Hit
AATTCATTAGTAATAGTTACGCTGCGGCCTTTTACACATGACCTTCGTGA54770.1844774223145264No Hit
AATTCACTTCCGGCGTGAATGTTACTGAATCCCCGATCATCTATCGCGAC47120.1587105375106899No Hit
AATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAG45920.15466867322773514No Hit
AATTCTGCCGTCACTGCATAAACCATCGGGAGAGCAGGCGGTACGCATAC43420.14624812263824608No Hit
AATTCTCTGACGAATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTT40600.1367497415733024No Hit
AATTCGCTTGCTGAGGTTTGCACCGGTGTGGCTCCGGAAGTTAACGCTAA39180.1319668688384726No Hit
AATTCGAATAAACCAATGGACAGAATCACCGATTCTCAACTTAGCGAGAT31540.10623366623699404No Hit
AATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATT31330.10552633998747697No Hit
AATTCACAACCTGACCCAGCAAAAGGTACACAATACTTTTATATTGGACT31170.10498742474974966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)