FastQCFastQC Report
Sun 5 Mar 2023
EGAF00001275643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001275643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67101247
Sequences flagged as poor quality0
Sequence length101
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG26138873.895437293438079TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC17603932.6234877572394444TruSeq Adapter, Index 12 (100% over 50bp)
TGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT2623320.3909495154389605Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1074420.16011922997496603TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATG1027060.15306123893643883TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATG845890.12606174070058637TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATG805230.1200022407929319TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATG773180.11522587650271239TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATG763280.11375049408545268TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATG721720.10755686850350188TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC720030.10730501029287876TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC687430.10244668031281147TruSeq Adapter, Index 12 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)