FastQCFastQC Report
Thu 16 Mar 2023
EGAF00001275644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001275644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67101247
Sequences flagged as poor quality0
Sequence length101
%GC23

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC25349293.7777673490926333Illumina Single End PCR Primer 1 (100% over 50bp)
AATAATACAACAACCACCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1209740.18028577024805514Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCC899860.13410481030255667Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCC859550.12809747037935076Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCC850220.12670703422247875Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGAGAAAGAGTGTAGATCTCGGTGGTCGCC820300.12224810069476055Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)