FastQCFastQC Report
Sun 14 May 2023
EGAF00001276391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001276391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45141759
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC11262702.4949625910678406TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCNCACGTCTGAACTCCAGTNACCGATGTATCTCGTATGC1095690.24272204368465128Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GATCGGAAGAGCGCACGTCTGAACTCCAGTNACCGATGTATCTCGTATGC508400.11262299282577802Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)