FastQCFastQC Report
Sat 13 May 2023
EGAF00001281636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001281636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40877040
Sequences flagged as poor quality0
Sequence length49-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC12379703.0285216346389072TruSeq Adapter, Index 5 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5259431.2866464890804226No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050440.5016116626839908No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1571690.3844921256529338TruSeq Adapter, Index 5 (100% over 49bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1378900.3373287302603124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)