FastQCFastQC Report
Sat 13 May 2023
EGAF00001281637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001281637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40877040
Sequences flagged as poor quality0
Sequence length49-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10748322.6294271796588014No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1326030.3243948191943448Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1325680.32430919655630647No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGG1301980.3185113207805653No Hit
ATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG726970.17784311192786953No Hit
ATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT628920.15385654147169167No Hit
ATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA571410.13978751886144397No Hit
ATGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCC537800.13156529924867358No Hit
ATGGGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT484280.11847237471206332No Hit
ATGGGGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTG479550.11731524591800188No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG462830.1132249301808546Illumina Single End PCR Primer 1 (100% over 50bp)
ATGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT422170.10327802600188273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)