FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001281646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001281646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24770454
Sequences flagged as poor quality0
Sequence length49-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT13027135.259140587411115TruSeq Adapter, Index 19 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5396162.178466329280844No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA1518840.6131659920322816TruSeq Adapter, Index 19 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1477190.5963516050210464No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT997390.40265309630578433No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT903810.3648742166776596TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)