FastQCFastQC Report
Thu 5 Jan 2023
EGAF00001281648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001281648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34130500
Sequences flagged as poor quality0
Sequence length49-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5203981.5247300801336048TruSeq Adapter, Index 12 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3498961.0251710347050291No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1901230.5570472158333456TruSeq Adapter, Index 12 (100% over 49bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT892300.261437716997993No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT639530.18737785851364616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)