FastQCFastQC Report
Sun 14 May 2023
EGAF00001281652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001281652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42305825
Sequences flagged as poor quality0
Sequence length49-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7518771.7772422591924397No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2827020.6682342207012865TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1453580.3435886192976972No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1269730.3001312467018431No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1026320.24259543455304322TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)