FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001281744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001281744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26009741
Sequences flagged as poor quality0
Sequence length49-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3343991.2856683194192513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2249560.8648913497446975TruSeq Adapter, Index 6 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT854960.32870761765755374No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420250.16157408103371734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)