FastQCFastQC Report
Tue 24 Jan 2023
EGAF00001283234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140978613
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG52748523.741597315899256TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC36164762.5652656974288717TruSeq Adapter, Index 12 (100% over 50bp)
TGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT5353520.379739868770024Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2153480.15275224760510306TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATG2071040.14690455211103545TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATG1739020.12335346213116737TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATG1616010.11462802517428654TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATG1598450.11338244617288157TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATG1540850.10929672006348935TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATG1467850.10411862968179436TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1465410.10394555378410483TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC1415340.10039395124422171TruSeq Adapter, Index 12 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)