FastQCFastQC Report
Tue 24 Jan 2023
EGAF00001283476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135669702
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1474840410.870816241639567TruSeq Adapter, Index 12 (100% over 49bp)
TGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT23729521.7490655356492197Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC19490131.4365867774958332TruSeq Adapter, Index 12 (100% over 50bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG10313820.7602154237797323TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATG2410990.1777102746197526TruSeq Adapter, Index 12 (97% over 49bp)
AGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT2391750.17629212453050128Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT2333040.17196470292239605Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATG2327390.1715482503234215TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATG2230030.1643719981046321TruSeq Adapter, Index 12 (97% over 49bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2137510.1575524946608934TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATG2044030.15066223112954136TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2022510.1490760258322083TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATG2000290.14743822463765713TruSeq Adapter, Index 12 (97% over 49bp)
CGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1958330.14434541914155602Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1631020.1202199146866262TruSeq Adapter, Index 12 (97% over 49bp)
AAGTAGAAGATGGTATATGAGATTATAAGGTGATTGGAGTTTAGATGTGT1588910.1171160529268355No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATG1575060.11609519124616341TruSeq Adapter, Index 12 (97% over 49bp)
TAAGTAGAAGATGGTATATGAGATTATAAGGTGATTGGAGTTTAGATGTG1479760.10907077838204436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)