FastQCFastQC Report
Mon 23 Jan 2023
EGAF00001283612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180492468
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG131161747.266881629653376TruSeq Adapter, Index 6 (100% over 49bp)
TGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT14765140.818047432317231Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC12513390.6932915339159749TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATG2437010.13502003862011572TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATG2264390.12545620463231738TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATG2097290.11619820058086855TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATG2070390.11470783368090463TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATG2001050.1108661221253842TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATG1994510.11050378013557885TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATG1934850.10719837904814952TruSeq Adapter, Index 6 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)