FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001283637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24695886
Sequences flagged as poor quality0
Sequence length126
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG3924041.5889448145330765No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC2463010.9973361555037953No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT2252560.9121195327837195No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA1583970.6413902299354637No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1569740.6356281366054248No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC1360420.5508690799755068No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA1343990.5442161500097628No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG1183280.47914053377149535No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA991260.40138669250416853No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA974300.39451915189436815No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA819340.3317718586812395No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC812360.3289454769915929No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG810730.32828544802968396No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG765940.3101488239782124No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA716320.29005640858562437No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG714270.28922631081144445No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA675500.2735273397358572No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA653330.26455013600240945No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC646870.26193431569938413No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT638500.2585450872262692No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT631240.2556053263284419No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT603760.24447796689699652No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC601760.24366811540999178No Hit
CGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATA589650.23876446465617798No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA589120.2385498540121217No Hit
CTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCA570930.23118425473781343No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT562940.22794889804722943No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA550260.22281443961961922No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN550010.22271320818374363No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG545160.2207493183277571No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC512720.20761352720853993No Hit
CTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCC511910.207285537356303No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAT509390.206265124482677No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT504970.2044753526963965No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA499290.20217537447330294No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC496050.20086341506435523No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA486120.1968425024313766No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA465750.18859416503623316No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC465190.18836740661987184No Hit
TCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTC451320.1827510865574938No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA450920.18258911626009286No Hit
GTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAA444790.1801069214524233No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG443080.17941449843103421No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG431210.17460802985566098No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA425530.17230805163256746No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC421230.17056687093550724No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG416510.16865562142617602No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA416340.1685867840497806No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC413400.1673963023638836No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG412460.16701567216499139No Hit
CAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT403120.16323366572067916No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA402490.16297856250227263No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC398020.161168544428817No Hit
CGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGG398000.16116044591394696No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC386730.15659693278467515No Hit
CAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCG384630.15574658872332015No Hit
CACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCA377100.15269749787474723No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC376820.15258411866656657No Hit
GTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGAC376650.15251528129017117No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG371540.150446110740874No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA369190.14949453524364342No Hit
CTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGG367660.14887499885608477No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCT362470.14677343424730743No Hit
CGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA361450.146360409988935No Hit
CCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCG360820.14610530677052852No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA357610.14480549513388585No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG354820.1436757523095142No Hit
CTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAG350840.14206414785037474No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG341340.13821735328710216No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC340960.13806348150457126No Hit
CAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAG340210.13775978719694448No Hit
CAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGC329690.13349996837529943No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTC328130.13286828421543573No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG326830.13234188074888262No Hit
CCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCG325290.13171829510388897No Hit
TGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCAC322800.13071003000256803No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG321760.13028890722932557No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT318990.12916726291982397No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA316410.12812255450158783No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT314910.12751516588633427No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTT314820.12747872256941906No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC313670.1270130579643913No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGA310320.12565655672365836No Hit
CTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTT308670.12498842924687942No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG303820.12302453939089288No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA303770.12300429310371777No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGT301920.12225518047823836No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT301530.12209725943827243No Hit
ACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTC299270.12118212725795706No Hit
GAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAC299160.1211375854261718No Hit
GTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGAT298600.12091082700981047No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT293000.11864324284619712No Hit
CGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATG292940.118618947301587No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT288370.11676843665378112No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTG286390.11596668368164642No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGT285480.11559820125505924No Hit
CTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCC285240.11550101907661867No Hit
TTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC284120.11504750224389601No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT280350.11352093219089204No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC279360.1131200557048247No Hit
GATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCC278640.11282850916950297No Hit
CCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTC278510.11277586882284765No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA277660.11243168194087065No Hit
CGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGA277110.11220897278194433No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC276860.11210774134606874No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT275830.11169066783026128No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC275630.1116096826815608No Hit
CACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAG270170.10939878812203782No Hit
CCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGT269720.10921657153746174No Hit
CGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATA269500.10912748787389123No Hit
ATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG268840.10886023688317964No Hit
CAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTC267960.10850390222889757No Hit
CGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGG266340.10784792252442371No Hit
GTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGC265990.10770619851419787No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA265020.10731342054300055No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT258750.10477453613124066No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG257030.10407806385241654No Hit
CATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAG255590.10349497078177314No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC254490.10304955246392052No Hit
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCT250020.10123953439046487No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT249210.10091154453822795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)