FastQCFastQC Report
Thu 5 Jan 2023
EGAF00001283655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68354037
Sequences flagged as poor quality0
Sequence length101
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTNNNGAAAGAGTGTAGATCTCGGTGGTCGCC66679489.755017103086391Illumina Single End PCR Primer 1 (96% over 28bp)
AATAATACAACAACCACCAANNNCTACACTCTTTCCCTACACAACACTCT1769710.25890350850821Illumina Single End PCR Primer 1 (95% over 22bp)
AGATCGGAAGAGCGTCGTGTNNNAAAAGAGTGTAGATCTCGGTGGTCGCC1058590.15486868756559324Illumina Single End PCR Primer 1 (100% over 26bp)
AGATCGGAAGAGCGTCGTGTNNNGAAAAAGTGTAGATCTCGGTGGTCGCC1047550.1532535671594642Illumina Single End PCR Primer 1 (100% over 22bp)
AGATCGGAAGAGCATCGTGTNNNGAAAGAGTGTAGATCTCGGTGGTCGCC929240.13594515273472435Illumina Single End PCR Primer 1 (100% over 27bp)
AGATCGGAAGAGCGTCGTATNNNGAAAGAGTGTAGATCTCGGTGGTCGCC831310.12161827398724087Illumina Single End PCR Primer 1 (100% over 27bp)
AGATCGGAAGAGCGTCATGTNNNGAAAGAGTGTAGATCTCGGTGGTCGCC826210.1208721585822356Illumina Single End PCR Primer 1 (100% over 27bp)
AATAATACAACAACCACCAANNNCGGAAGAGCGTCGTGTAGGGAAAGAGT770910.11278192683776672Illumina Single End PCR Primer 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)