FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001283701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001283701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35850184
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG29617278.261399718338962RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC22642696.31592016375704RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT12020713.3530399732397473RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG10969063.0596941985011847RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG10587952.953387910087156RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG7238862.019197446796926RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG7129111.988583935859297RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6681131.8636250235145237RNA PCR Primer, Index 1 (100% over 31bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC6616181.845507961688565RNA PCR Primer, Index 1 (100% over 51bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG5615901.5664912626389866RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG5010661.3976664666490972RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG4635471.2930114947248248RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA3727151.039645988985719RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT3306220.9222323656693087RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC3213570.896388704727429RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA3188020.8892618235934298RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG3116140.8692117172955096RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA2936190.8190167169016483RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2878900.803036324722908RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2866140.799477068234852RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2471570.6894162663153974RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2454140.6845543665828884RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2302790.6423370100415663RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2175320.6067807071785183RNA PCR Primer, Index 1 (100% over 30bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2095750.5845855630754921RNA PCR Primer, Index 1 (100% over 29bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2094180.5841476294793913RNA PCR Primer, Index 1 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1966900.5486443249496292RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1935330.5398382334662494RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA1926890.5374839917139617RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1855980.5177044558543967RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1809260.504672444637941RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC1797600.501420020605752RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1760370.4910351366676388RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCCA1713410.4779361801880849RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1657990.4624774031843184RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1657380.46230725064061035RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1650000.4602486838003398RNA PCR Primer, Index 1 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1644410.4586894170473435Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1462170.40785564726808654RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA1417650.3954373009633647RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1327310.37023798817880543RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC1322660.3689409237062772RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1297350.36188098783537626RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1254130.3498252617057698RNA PCR Primer, Index 1 (100% over 29bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA1241880.3464082639017976RNA PCR Primer, Index 1 (100% over 23bp)
TGAGAACTGAATTCCATGGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA1210900.33776674619020086RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1202570.33544318768349973RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1149240.3205673923458803RNA PCR Primer, Index 1 (100% over 22bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAAC1144630.3192814854172018RNA PCR Primer, Index 1 (100% over 24bp)
TGAGAACTGAATTCCATGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA1124000.3135269821767163RNA PCR Primer, Index 1 (100% over 28bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC1082740.3020179756957454RNA PCR Primer, Index 1 (100% over 24bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1068710.29810446719046124RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1048340.29242248798499887RNA PCR Primer, Index 1 (100% over 29bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGGA1011320.2820961811520967RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC971710.27104742335492615RNA PCR Primer, Index 1 (100% over 24bp)
TGAGAACTGAATTCCATGGGTAATGGAATTCTCGGGTGCCAAGGAACTCCA943390.2631478823093349RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT926490.2584338200328344RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA926470.2584282412609096RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA919060.25636130626275166RNA PCR Primer, Index 1 (100% over 37bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC906350.25281599670450783RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA896930.2501883951279023RNA PCR Primer, Index 1 (100% over 23bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGGA866250.24163055899517838RNA PCR Primer, Index 1 (100% over 22bp)
CAAAGAATTCTCCTTTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT860650.24006850285621964RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC814410.22717038216596044RNA PCR Primer, Index 1 (100% over 31bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC811390.22632798760530767RNA PCR Primer, Index 1 (100% over 33bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA796470.22216622374936762RNA PCR Primer, Index 1 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA786600.2194130998044529RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC779020.21729874524493376RNA PCR Primer, Index 1 (100% over 31bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAG778250.2170839625258269RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA764660.2132931870028896RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC754600.21048706472468873RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT738070.20587620972879805RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA712460.1987325922790243RNA PCR Primer, Index 1 (100% over 32bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT687010.19163360500464935RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC647700.18066852878635156RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC623750.17398794940634055RNA PCR Primer, Index 1 (100% over 33bp)
TGAGAACTGAATTCCATGGGTTAATGGAATTCTCGGGTGCCAAGGAACTCC607220.16937709441044987RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT593510.16555284625596342RNA PCR Primer, Index 1 (100% over 35bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA592460.16525996072990867RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA580840.1620186942415693RNA PCR Primer, Index 1 (100% over 28bp)
TGGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG577150.160989410821434Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACTC568650.15861843275337162RNA PCR Primer, Index 1 (100% over 26bp)
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAAC555790.15503128240569142RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC552000.15397410512593185RNA PCR Primer, Index 1 (100% over 27bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG548770.15307313346006815RNA PCR Primer, Index 1 (100% over 29bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG544540.1518932231979618No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG539100.15037579723440192RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT534330.14904526013032457RNA PCR Primer, Index 1 (100% over 35bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG519570.14492812644978337RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG498020.1389169997007547RNA PCR Primer, Index 1 (100% over 29bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA487870.136085772948892RNA PCR Primer, Index 1 (100% over 22bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAGT478940.1335948512844453RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA477840.13328801882857838RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC476020.1327803505834168RNA PCR Primer, Index 1 (100% over 31bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA468790.13076362453258258RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC460380.12841775093818206RNA PCR Primer, Index 1 (100% over 31bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC460040.12832291181545957RNA PCR Primer, Index 1 (100% over 33bp)
TGAGAACTGAATTCCATAGGCTGATGGAATTCTCGGGTGCCAAGGAACTCC455210.12697563839560766RNA PCR Primer, Index 1 (100% over 27bp)
CACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGGAA443610.12373995067919318RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA434510.12120160945338523RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGGA432770.12071625629592306RNA PCR Primer, Index 1 (100% over 22bp)
TGAGAACTGAATTCCATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCCA429430.11978460138447267RNA PCR Primer, Index 1 (100% over 28bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGAA404460.1128195046362942RNA PCR Primer, Index 1 (100% over 23bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG403760.11262424761892435RNA PCR Primer, Index 1 (100% over 29bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC400540.1117260653390231RNA PCR Primer, Index 1 (100% over 31bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT396670.1106465729715641RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG395190.11023374384912502RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCCA394150.10994364770903267RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGATGGAATTCTCGGGTGCCAAGGAACTCCA385170.10743877911477387RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA382650.10673585385224243RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT372770.10397994052136525RNA PCR Primer, Index 1 (100% over 30bp)
TACAGTACTGTGATAACTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC372230.10382931367939423RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC372220.10382652429343181RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph